TeloBase is a curated database containing computationally predicted and experimentally confirmed telomere sequences of species across the whole tree of life. It contains information from the literature (1978 – September 2022) and predicted telomere motifs from publicly available NGS data stored in the SRA database (NCBI) and user submitted data. TeloBase is supplemented by internal taxonomy utilizing popular on-line taxonomic resources that enables in-house data filtration and graphical visualisation of telomere DNA evolutionary dynamics in the form of heat tree plots. Its manual and other relevant data (raw results from NGS data analysis and Atlas of telomere sequences) can be accessed on https://www.ceitec.eu/chromatin-molecular-complexes/rg51/tab?tabId=125#TeloBase.