Unlocking the mysteries surrounding nutrient fate within the human body has remained an enigmatic pursuit. Our organs and tissues crave specific nutrients, especially in physiological and pathological conditions. Yet, the intricate journey these nutrients embark upon within our bodies remains largely unclear. The aim of my ambitious project is thus to create an innovative chemical-spatial workflow capable of (1) providing the anatomical coordinates of nutrients of interest across tissues (and organs) and (2) understanding the biochemical route these nutrients undergo in the respective tissue areas of the target organ. For this, I will use a complementary approach relying on advanced techniques such as tracer metabolomics, mass spectrometry imaging (MSI) and Stimulated Raman spectroscopy (SRS). I will benchmark this technological platform onto a model of acute kidney injury (AKI) to identify and localize the predominant biochemical pathways. The amalgamation of SRS and MSI with tracer metabolomics is groundbreaking given the current technological landscape and provides an innovative and feasible solution to the posed challenge.