Floods and droughts put increasing pressure on the European society, with estimated annual damages from river flooding of €7.6 billion and drought-induced losses over €9 billion. Fostering sponge measures as nature-based solutions to boost the natural retention function of landscapes is promising but implementation must be rapidly upscaled and innovative approaches to increase the integration, involvement and cooperation of diverse actors from local to basin wide levels are urgently needed. SpongeWorks aims to demonstrate practical, effective, economically feasible and inclusive approaches and solutions towards enhancing the sponge functioning of interconnected groundwater, soil and surface water systems at regional scale. It applies an integrative multi-actor approach to demonstrate the effectiveness of multifunctional sponge measures for improved water and soil management for enhancing water retention in three large demonstrators in the Pinios (GR), Lèze (FR) and Vecht (NL/DE) river basins. In each demonstrator, SpongeWorks evaluates existing sponge measures, draws lessons-learned and best practices, and implements new sponge measures. The effectiveness of large-scale implementation is assessed to co-create long-term sponge strategies with action plans and roadmaps at landscape scale. Broad dissemination of best-practices, promotion of exemplary demonstration areas as lighthouses, and targeted replication in eight associated regions ensures long-lasting impact of this work. SpongeWorks contributes to transformative change of landscape management to ensure regions are more adapted and resilient to climate change impacts on soils, waters, habitats and biodiversity. By combining scientific excellence and practical know-how from established communities of practice, as well as facilitating mutual learning and sharing of successful strategies, SpongeWorks will catalyse a shift in the pace of implementation of sponge measures and strategies across Europe and beyond.